ch10f6Tran­scrip­tion of two genes. (a) RNA poly­merase moves from the 3′ end of the tem­plate strand, cre­at­ing an RNA strand that grows in a 5′ → 3′ direc­tion (because it must be antipar­al­lel to the tem­plate strand). Note that some genes are tran­scribed from one strand of the DNA dou­ble helix; oth­er genes use the oth­er strand as the tem­plate. (b) A uracil is being added to the 3′ end of the tran­script for gene 1. Growth is thus 5′ → 3′.

from: NCBI

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