Amplicon Sequencing



Ampli­con sequenc­ing is a high­ly tar­get­ed approach for ana­lyz­ing genet­ic vari­a­tion in speci­fic genomic regions. The ultra-deep sequenc­ing of PCR prod­ucts (ampli­cons) allows effi­cient vari­ant iden­ti­fi­ca­tion and char­ac­ter­i­za­tion. Illu­mi­na ampli­con tech­nol­o­gy uses a pair of oligonu­cleotide probes designed to tar­get and cap­ture regions of inter­est, fol­lowed by next-gen­er­a­tion sequenc­ing (NGS).

Ampli­con sequenc­ing is use­ful for the dis­cov­ery of rare somat­ic muta­tions in com­plex sam­ples (such as tumors mixed with germline DNA). Anoth­er com­mon appli­ca­tion is sequenc­ing the bac­te­ri­al 16S rRNA gene across mul­ti­ple species, a wide­ly used method for phy­logeny and tax­on­o­my stud­ies, par­tic­u­lar­ly in diverse metage­nomics sam­ples.


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