Key points of the analysis of microarray — 基因芯片分析要点

  1. bio­log­i­cal repli­cates — 生物学重复
    Five or more is usu­al­ly robust for micro-array stud­ies
    五个及以上的样本数对基因芯片研究来说才是具有鲁棒性的。
  2. qPCR val­i­da­tion
    Micro-array may give many false pos­i­tives so it is usu­al­ly nec­es­sary to val­i­date the dif­fer­en­tial expres­sion observed in some of the key genes.
    基因芯片可能产生许多的假阳性结果,所以验证部分关键的差异表达基因通常来说是必须的。

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